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hog cholera 豬霍亂。

hog deer

Since the important roles of eo protein in the viral infection . immunity and virus - host interaction . the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study , which will provide some evidence for epidemiological study . in addition , the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems , and the recombinant proteins were preliminarily analyzed by immunological method 鑒于eo蛋白在病毒感染,誘導機體免疫及與宿主細胞相互關系中的作用,本研究克隆了2株豬瘟病毒eo基因并將其與其它毒株eo基因進行了序列分析,揭示了我國豬瘟病毒流行株之間的遺傳演化關系,為豬瘟病毒的流行病學研究提供依據。

A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv 以豬瘟兔化弱毒犢牛睪丸細胞毒( hclv )中提取的細胞總rna為模板,以rt - pcr技術,擴增出了完整e2基因的cdna片段。經電泳、酶切分析,證實了所擴增片段為e2基因特異性片段。

Hc ( hog cholera ) , the virulent infectious disease caused by hcv ( hog cholera virus ) , had been controlled effectively by hclv strain vaccine in the country 豬瘟是由豬瘟病毒引起的豬的一種病毒性傳染病。豬瘟兔化弱毒疫苗有效地控制了豬瘟在我國的急性發生和大流行。

The highest antiviral litre of rpoifn - a was l l08iu / mi . the rpoifn - a protected pk - 15 cells against virulent hog cholera virus ( hcv ) infection in vitro Sds - page分析表明,構建的重組表達質粒pqe30 poifn在大腸桿菌jm109中表達的rpoifn占菌體蛋白總量的20 26 。